Fluorescence is a powerful spectroscopic technique at the interface of chemistry, physics, and light–matter interactions. It is based on the ability of atoms and molecules, whether organic or inorganic, to absorb light and then emit another light as they return from an excited electronic state to the ground state.
Because fluorescence arises from electronically excited states, it provides valuable information about the structure, environment, and electronic properties of molecular systems. By measuring the emitted light, scientists can investigate processes such as energy transfer, charge transfer, molecular binding, and changes in polarity, viscosity, or local environment.
Fluorescence is also one of the most sensitive analytical techniques available, it’s roughly 1000 times more sensitive than absorption spectrophotometric methods. Its Limit Of Detection (LOD) can reach the picograms per liter of concentration. This high sensitivity among other properties make fluorescence a key tool for the study and development of photoluminescent and photoactive materials, including:
- Organic Light Emitting Diodes (OLEDs)
- Photovoltaic Cells (PVs)
- Imaging probes
- Chemical and biological sensors
- Environment-sensitive fluorophores for polarity, pH, temperature, morphology, and size-related studies.
In addition to materials research, fluorescence can be used as a selective detection method in chemical, environmental, and food analysis when the target analyte is naturally fluorescent or can be derivatized with a fluorescent probe.
At LuminoChemi, fluorescence is one of our core areas of expertise. We support clients with photophysical investigations, method development, training and research project design involving fluorescence-based approaches. We perform steady-state and time-resolved measurements in solution and in the solid state, including polymer matrices such as PMMA and polystyrene or KBr pellets.
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